A new, simple, accurate and precise validated RP-HPLC method for estimation of Embelin has been developed and validated. Analysis was carried out on LC-2010 CHT Shimadzu system with ACE C18 column (5µ, 150mm) using Methanol: water (85:15) as mobile phase with flow rate of 1.2 ml/min. The detection was carried out using UV detector set at 288 nm. For this method, Beer’s law is obeyed in the concentration range of 50.0 to 250.0 ppm of embelin. The method was validated with respect to linearity, precision and accuracy as per the International Conference on Harmonization (ICH Q2-R1) guidelines.
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